Isolation of Extracellular Polymeric Substances from Biofilms of the Thermoacidophilic Archaeon Sulfolobus acidocaldarius
نویسندگان
چکیده
منابع مشابه
Isolation of Extracellular Polymeric Substances from Biofilms of the Thermoacidophilic Archaeon Sulfolobus acidocaldarius
Extracellular polymeric substances (EPS) are the major structural and functional components of microbial biofilms. The aim of this study was to establish a method for EPS isolation from biofilms of the thermoacidophilic archaeon, Sulfolobus acidocaldarius, as a basis for EPS analysis. Biofilms of S. acidocaldarius were cultivated on the surface of gellan gum-solidified Brock medium at 78°C for ...
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cis-polyprenyl diphosphate synthases are involved in the biosynthesis of the glycosyl carrier lipid in most organisms. However, only little is known about this enzyme of archaea. In this report, we isolated the gene of cis-polyprenyl diphosphate synthase from a thermoacidophilic archaeon, Sulfolobus acidocaldarius, and characterized the recombinant enzyme.
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Department of Biochemistry and Molecular Biology, Royal Free Hospital School of Medicine, University of London, Rowland Hill Street, London NW3 ZPF, UK Growth of the thermoacidophilic archaeon Sulfolobus shibatae was tested on a range of carbon and nitrogen sources. Optimal defined and complex growth media were developed and growth conditions in both shake flask and fermenter cultures were opti...
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ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2015
ISSN: 2296-4185
DOI: 10.3389/fbioe.2015.00123